Journal: bioRxiv

Article Title: Evolution of sex hormone-binding globulin gene expression in the primate testis

doi: 10.1101/2024.08.05.606716

Figure Lengend Snippet: RT-PCR assays for SHBG transcripts (exons 1A-8 and 1-8) were performed on cDNA derived from HepG2 cells, rhesus macaque, lion-tailed macaque and baboon testes. Analyses for TNP1 and Vimentin transcripts indicate the presence of germ and Sertoli cells, respectively, in testis-derived cDNA. The integrity of all cDNA samples is assessed by analysis for GAPDH transcripts. All PCR products were subjected to electrophoresis on either 1% (for SHBG ) or 1.5% (for TNP1 , Vimentin and GAPDH ) agarose gels. Molecular sizes in bp are indicated for the SHBG assays.

Article Snippet: Non-human primate testis cDNA samples were a generous gift from Dr. Rüdiger Behr at the Deutsches Primatenzentrum in Göttingen, Germany.

Techniques: Reverse Transcription Polymerase Chain Reaction, Derivative Assay, Electrophoresis

Journal: bioRxiv

Article Title: Evolution of sex hormone-binding globulin gene expression in the primate testis

doi: 10.1101/2024.08.05.606716

Figure Lengend Snippet: RT-PCR assays for SHBG transcripts (exons 3-8) were performed on cDNA derived from HepG2 cells, and three marmoset testes. Analyses for TNP1 and Vimentin transcripts indicate the presence of germ and Sertoli cells, respectively, in testis-derived cDNA. The integrity of all cDNA samples is assessed by analysis for GAPDH transcripts. All PCR products were subjected to electrophoresis on either 1% (for SHBG ) or 1.5% (for TNP1 , Vimentin and GAPDH ) agarose gels. Molecular sizes in bp are indicated for the SHBG assay.

Article Snippet: Non-human primate testis cDNA samples were a generous gift from Dr. Rüdiger Behr at the Deutsches Primatenzentrum in Göttingen, Germany.

Techniques: Reverse Transcription Polymerase Chain Reaction, Derivative Assay, Electrophoresis

Journal: bioRxiv

Article Title: Evolution of sex hormone-binding globulin gene expression in the primate testis

doi: 10.1101/2024.08.05.606716

Figure Lengend Snippet: RT-PCR assays for SHBG transcripts (exons 3-8) were performed on cDNA derived from liver and testes from two grey mouse lemurs ( Microcebus murinus ). Analyses for TNP1 and Vimentin transcripts indicate the presence of germ and Sertoli cells, respectively, in testis-derived cDNA. The integrity of all cDNA samples is assessed by analysis for GAPDH transcripts. All PCR products were subjected to electrophoresis on either 1% (for SHBG ) or 1.5% (for TNP1 , Vimentin and GAPDH ) agarose gels. Molecular sizes in bp are indicated for the SHBG assay. RT, reverse transcriptase.

Article Snippet: Non-human primate testis cDNA samples were a generous gift from Dr. Rüdiger Behr at the Deutsches Primatenzentrum in Göttingen, Germany.

Techniques: Reverse Transcription Polymerase Chain Reaction, Derivative Assay, Electrophoresis, Reverse Transcription

Journal: Scientific Data

Article Title: Small non-coding RNA transcriptomic profiling in adult and fetal human brain

doi: 10.1038/s41597-024-03604-6

Figure Lengend Snippet: Characterization of small non-coding RNA (sncRNA) expression profiles in the adult and fetal human brain. ( a ) Brain regions subjected to sncRNA transcriptomic profiling and schematic overview of the sncRNA-seq analysis workflow and filtering strategy. The brain macroregions are indicated by different colors: Brain Stem (sea green), Cerebellum (sky blue), Cerebral cortex (black), Corpus Callosum (medium purple), Limbic system (dark orange), Meninges (olive drab). For details see Table . ( b ) Abundance of detected sncRNA species per sample, including samples from both the brain and testis. ( c ) Percentage representation of the average expression across all sncRNA populations per sample. ( d ) Circos plot illustrating genomic positions and mean log2 (count + 1) expression levels of miRNAs (blue), piRNAs (orange), and snoRNAs (yellow) detected (reads ≥ 10) in the adult and fetal human brain. Samples that are pools of RNA from different individuals are indicated as pools. M: mitochondrial genome.

Article Snippet: As a control for germline piRNAs, 3 human testis RNA samples sourced from BioChain Institute (USA) were also included in the study, two of them were pools of 5 and 39 different individuals (Table ).

Techniques: Expressing

Journal: Scientific Data

Article Title: Small non-coding RNA transcriptomic profiling in adult and fetal human brain

doi: 10.1038/s41597-024-03604-6

Figure Lengend Snippet: Differential expression analysis. Heatmaps showing differentially expressed miRNAs ( a ), piRNAs ( b ) and snoRNAs ( c ) between adult and fetal brains. Only sncRNAs with a read count ≥ 100 in at least one sample, with |FC| ≥ 2.0, and adjusted p-value ≤ 0.05 were considered. Expression levels of sncRNAs are displayed from yellow (high expression) to blue (low expression). Samples that are pools of RNA from different individuals are indicated as pool.

Article Snippet: As a control for germline piRNAs, 3 human testis RNA samples sourced from BioChain Institute (USA) were also included in the study, two of them were pools of 5 and 39 different individuals (Table ).

Techniques: Quantitative Proteomics, Expressing

Journal: Scientific Data

Article Title: Small non-coding RNA transcriptomic profiling in adult and fetal human brain

doi: 10.1038/s41597-024-03604-6

Figure Lengend Snippet: Validation of the PIWI-piRNA pathway activity in the human brain. ( a ) Expression of genes involved in piRNA biogenesis by RNA-Seq analysis of pooled brain and testis samples. The genes were categorized by their respective role in the piRNA pathway. ( b ) Real-time PCR quantification of PIWIL1 , PIWIL2 , PIWIL3 , and PIWIL4 gene expression in adult and fetal brain samples. ( c ) Heatmaps depicting expression (left) with respect to fold-change (FC) (right) of differentially expressed piRNAs in adult and fetal brain samples compared to the testis group. Only piRNAs with a read count ≥ 100 in at least one sample with |FC| ≥ 1.5 and adjusted p-value ≤ 0.05 were considered. Expression levels are displayed from yellow (high expression) to blue (low expression) while FCs are displayed from green (under-expressed) to red (over-expressed). Samples that are pools of RNA from different individuals are indicated as pool.

Article Snippet: As a control for germline piRNAs, 3 human testis RNA samples sourced from BioChain Institute (USA) were also included in the study, two of them were pools of 5 and 39 different individuals (Table ).

Techniques: Biomarker Discovery, Activity Assay, Expressing, RNA Sequencing, Real-time Polymerase Chain Reaction, Gene Expression

Journal: BMC Biochemistry

Article Title: Cloning and characterisation of hAps1 and hAps2, human diadenosine polyphosphate-metabolising Nudix hydrolases

doi: 10.1186/1471-2091-3-20

Figure Lengend Snippet: Tissue distribution of hAps1, hAps2 and β-actin. PCR using human tissue first-strand cDNA as template was performed with primers specific for β-actin and for hAps1 or hAps2. 35 cycles of amplification were used for β-actin, 40 cycles for hAsp1 and hAps2.

Article Snippet: The tissue distribution of hAps1 and hAps2 expression was determined by PCR from human tissue cDNA samples (human cDNA panels, Clontech) and human cDNA libraries (testis from Clontech, brain from Dr Peter Cheung of the MRC Protein Phosphorylation Unit, Wellcome Trust Biocentre, Dundee, Scotland, UK). β-Actin-specific and hAps1 and hAps2-specific primers were used.

Techniques: Amplification